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LCTL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LCTL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102758-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LCTL (NM_207338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KL lactase phlorizin hydrolase | Lactase-like protein | Lactase-like | LCTL | Klotho gamma | KLPH | KLG
Origin Species
Human
SwissProt
Q6UWM7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPVWVATLL WMLLLVPRLG AARKGSPEEA SFYYGTFPLG FSWGVGSSAY QTEGAWDQDG
61KGPSIWDVFT HSGKGKVLGN ETADVACDGY YKVQEDIILL RELHVNHYRF SLSWPRLLPT
121GIRAEQVNKK GIEFYSDLID ALLSSNITPI VTLHHWDLPQ LLQVKYGGWQ NVSMANYFRD
181YANLCFEAFG DRVKHWITFS DPRAMAEKGY ETGHHAPGLK LRGTGLYKAA HHIIKAHAKA
241WHSYNTTWRS KQQGLVGISL NCDWGEPVDI SNPKDLEAAE RYLQFCLGWF ANPIYAGDYP
301QVMKDYIGRK SAEQGLEMSR LPVFSLQEKS YIKGTSDFLG LGHFTTRYIT ERNYPSRQGP
361SYQNDRDLIE LVDPNWPDLG SKWLYSVPWG FRRLLNFAQT QYGDPPIYVM ENGASQKFHC
421TQLCDEWRIQ YLKGYINEML KAIKDGANIK GYTSWSLLDK FEWEKGYSDR YGFYYVEFND
481RNKPRYPKAS VQYYKKIIIA NGFPNPREVE SWYLKALETC SINNQMLAAE PLLSHMQMVT
541EIVVPTVCSL CVLITAVLLM LLLRRQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

567 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.