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LAS1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LAS1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G130152-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LAS1L (NM_001170650), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Las1-like | LAS1L | LAS1-like (S. cerevisiae) | DJ475B7.2 | Protein LAS1 homolog | RP3-475B7.2
Origin Species
Human
NCBI GenBank
NM_031206
SwissProt
Q9Y4W2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MSWESGAGPG LGSQGMDLVW SAWYGKCVKG KGSLPLSAHG IVVAWLSRAE WDQVTVYLFC
61DDHKLQRYAL NRITVWRSRF VNLISERKTK FAKVPLKCLA QEVNIPDWIV DLRHELTHKK
121MPHINDCRRG CYFVLDWLQK TYWCRQLENS LRETWELEEF REGIEEEDQE EDKNIVVDDI
181TEQKPEPQDD GKSTESDVKA DGDSKGSEEV DSHCKKALSH KELYERAREL LVSYEEEQFT
241VLEKFRYLPK AIKAWNNPSP RVECVLAELK GVTCENREAV LDAFLDDGFL VPTFEQLAAL
301QIEYEENVDL NDVLVPKPFS QFWQPLLRGL HSQNFTQALL ERMLSELPAL GISGIRPTYI
361LRWTVELIVA NTKTGRNARR FSAGQWEARR GWRLFNCSAS LDWPRMVESC LGSPCWASPQ
421LLRIIFKAMG QGLPDEEQEK LLRICSIYTQ SGENSLVQEG SEASPIGKSP YTLDSLYWSV
481KPASSSFGSE AKAQQQEEQG SVNDVKEEEK EEKEVLPDQV EEEEENDDQE EEEEDEDDED
541DEEEDRMEVG PFSTGQESPT AENARLLAQK RGALQGSAWQ VSSEDVRWDT FPLGRMPGQT
601EDPAELMLEN YDTMYLLDQP VLEQRLEPST CKTDTLGLSC GVGSGNCSNS SSSNFEGLLW
661SQGQLHGLKT GLQLF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

675 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.