Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LANO / LRRC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111898-100
Catalog Number: LS-G111898
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lano(LRRC1) (NM_018214), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LAP and no PDZ protein | LANO adapter protein | DJ523E19.1 | LANO | LRRC1
- Origin Species
- Human
- NCBI GenBank
- NM_018214
- SwissProt
- Q9BTT6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFHCIPLWRC NRHVESIDKR HCSLVYVPEE IYRYARSLEE LLLDANQLRE LPEQFFQLVK61LRKLGLSDNE IQRLPPEIAN FMQLVELDVS RNEIPEIPES ISFCKALQVA DFSGNPLTRL121PESFPELQNL TCLSVNDISL QSLPENIGNL YNLASLELRE NLLTYLPDSL TQLRRLEELD181LGNNEIYNLP ESIGALLHLK DLWLDGNQLS ELPQEIGNLK NLLCLDVSEN RLERLPEEIS241GLTSLTDLVI SQNLLETIPD GIGKLKKLSI LKVDQNRLTQ LPEAVGECES LTELVLTENQ301LLTLPKSIGK LKKLSNLNAD RNKLVSLPKE IGGCCSLTVF CVRDNRLTRI PAEVSQATEL361HVLDVAGNRL LHLPLSLTAL KLKALWLSDN QSQPLLTFQT DTDYTTGEKI LTCVLLPQLP421SEPTCQENLP RCGALENLVN DVSDEAWNER AVNRVSAIRF VEDEKDEEDN ETRTLLRRAT481PHPGELKHMK KTVENLRNDM NAAKGLDSNK NEVNHAIDRV TTSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
524 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.