Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LAMA4 / Laminin Alpha 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118064-100
Catalog Number: LS-G118064
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Laminin alpha 4(LAMA4) (NM_002290), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LAMA4 | Laminin alpha 4 chain | Laminin-8 subunit alpha | LAMA4*-1 | Laminin subunit alpha-4 | Laminin | alpha 4 | Laminin-14 subunit alpha | Laminin-9 subunit alpha
- Origin Species
- Human
- NCBI GenBank
- NM_002290
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 201.82 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALSSAWRSV LPLWLLWSAA CSRAASGDDN AFPFDIEGSS AVGRQDPPET SEPRVALGRL61PPAAEKCNAG FFHTLSGECV PCDCNGNSNE CLDGSGYCVH CQRNTTGEHC EKCLDGYIGD121SIRGAPQFCQ PCPCPLPHLA NFAESCYRKN GAVRCICNEN YAGPNCERCA PGYYGNPLLI181GSTCKKCDCS GNSDPNLIFE DCDEVTGQCR NCLRNTTGFK CERCAPGYYG DARIAKNCAV241CNCGGGPCDS VTGECLEEGF EPPTGCDKCV WDLTDDLRLA ALSIEEGKSG VLSVSSGAAA301HRHVNEINAT IYLLKTKLSE RENQYALRKI QINNAENTMK SLLSDVEELV EKENQASRKG361QLVQKESMDT INHASQLVEQ AHDMRDKIQE INNKMLYYGE EHELSPKEIS EKLVLAQKML421EEIRSRQPFF TQRELVDEEA DEAYELLSQA ESWQRLHNET RTLFPVVLEQ LDDYNAKLSD481LQEALDQALN YVRDAEDMNR ATAARQRDHE KQQERVREQM EVVNMSLSTS ADSLTTPRLT541LSELDDIIKN ASGIYAEIDG AKSELQVKLS NLSNLSHDLV QEAIDHAQDL QQEANELSRK601LHSSDMNGLV QKALDASNVY ENIVNYVSEA NETAEFALNT TDRIYDAVSG IDTQIIYHKD661ESENLLNQAR ELQAKAESSS DEAVADTSRR VGGALARKSA LKTRLSDAVK QLQAAERGDA721QQRLGQSRLI TEEANRTTME VQQATAPMAN NLTNWSQNLQ HFDSSAYNTA VNSARDAVRN781LTEVVPQLLD QLRTVEQKRP ASNVSASIQR IRELIAQTRS VASKIQVSMM FDGQSAVEVH841SRTSMDDLKA FTSLSLYMKP PVKRPELTET ADQFILYLGS KNAKKEYMGL AIKNDNLVYV901YNLGTKDVEI PLDSKPVSSW PAYFSIVKIE RVGKHGKVFL TVPSLSSTAE EKFIKKGEFS961GDDSLLDLDP EDTVFYVGGV PSNFKLPTSL NLPGFVGCLE LATLNNDVIS LYNFKHIYNM1021DPSTSVPCAR DKLAFTQSRA ASYFFDGSGY AVVRDITRRG KFGQVTRFDI EVRTPADNGL1081ILLMVNGSMF FRLEMRNGYL HVFYDFGFSG GPVHLEDTLK KAQINDAKYH EISIIYHNDK1141KMILVVDRRH VKSMDNEKMK IPFTDIYIGG APPEILQSRA LRAHLPLDIN FRGCMKGFQF1201QKKDFNLLEQ TETLGVGYGC PEDSLISRRA YFNGQSFIAS IQKISFFDGF EGGFNFRTLQ1261PNGLLFYYAS GSDVFSISLD NGTVIMDVKG IKVQSVDKQY NDGLSHFVIS SVSPTRYELI1321VDKSRVGSKN PTKGKIEQTQ ASEKKFYFGG SPISAQYANF TGCISNAYFT RVDRDVEVED1381FQRYTEKVHT SLYECPIESS PLFLLHKKGK NLSKPKASQN KKGGKSKDAP SWDPVALKLP1441ERNTPRNSHC HLSNSPRAIE HAYQYGGTAN SRQEFEHLKG DFGAKSQFSI RLRTRSSHGM1501IFYVSDQEEN DFMTLFLAHG RLVYMFNVGH KKLKIRSQEK YNDGLWHDVI FIRERSSGRL1561VIDGLRVLEE SLPPTEATWK IKGPIYLGGV APGKAVKNVQ INSIYSFSGC LSNLQLNGAS1621ITSASQTFSV TPCFEGPMET GTYFSTEGGY VVLDESFNIG LKFEIAFEVR PRSSSGTLVH1681GHSVNGEYLN VHMKNGQVIV KVNNGIRDFS TSVTPKQSLC DGRWHRITVI RDSNVVQLDV1741DSEVNHVVGP LNPKPIDHRE PVFVGGVPES LLTPRLAPSK PFTGCIRHFV IDGHPVSFSK1801AALVSGAVSI NSCPAATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1816 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.