LSBio
LACE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LACE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LACE1 antibodies →
$504.00each
Catalog Number: LS-G106678-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LACE1 (NM_145315), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATPase family gene 1 homolog | CG8520 gene product | AFG1 | LACE1 | Lactation elevated 1 | Protein AFG1 homolog | Lactation elevated protein 1
Origin Species
Human
NCBI GenBank
NM_145315
SwissProt
Q8WV93

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASWSLLVT LRPLAQSPLR GRCVGCGAWA AALAPLATAP GKPFWKAYTV QTSESMTPTA
61TSETYLKALA VCHGPLDHYD FLIKAHELKD DEHQRRVIQC LQKLHEDLKG YNIEAEGLFS
121KLFSRSKPPR GLYVYGDVGT GKTMVMDMFY AYVEMKRKKR VHFHGFMLDV HKRIHRLKQS
181LPKRKPGFMA KSYDPIAPIA EEISEEACLL CFDEFQVTDI ADAMILKQLF ENLFKNGVVV
241VATSNRPPED LYKNGLQRAN FVPFIAVLKE YCNTVQLDSG IDYRKRELPA AGKLYYLTSE
301ADVEAVMDKL FDELAQKQND LTRPRILKVQ GRELRLNKAC GTVADCTFEE LCERPLGASD
361YLELSKNFDT IFLRNIPQFT LANRTQGRRF ITLIDNFYDL KVRIICSAST PISSLFLHQH
421HDSELEQSRI LMDDLGLSQD SAEGLSMFTG EEEIFAFQRT ISRLTEMQTE QYWNEGDRTK
481KTRTRPLEQK LISEEDLAAN DILDYKDDDD KV

512 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.