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L3MBTL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

L3MBTL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105348-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human L3MBTL4 (NM_173464), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
H-l(3)mbt-like protein 4 | HsT1031 | L(3)mbt-like 4 (Drosophila) | L3MBTL4 | L(3)mbt-like protein 4
Origin Species
Human
SwissProt
Q8NA19

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKQPNRKRKL NMDSKERLDQ DGRLEQAEEE KKPKDSTTPL SHVPSAAAQG AWSWEWYLKE
61QKAVAAPVEL FSKDQSFPEH ENGFQIGMRL EGIDPRHPSV FCVLSVAEVC GYRLRLHFDG
121YLSCYDFWTN AGSPDIHPVG WCEKTKHELH IPKGYRKDKF VWMDYLKACK LQNAPKKLFR
181NRSPNGPMSK EFQVGMKLEA VDRKNPSLVC VATIADIVED RLLVHFDNWD DSYDYWCDVN
241SPYVQPVGWC QENGRTLIAP QGYPNPENFS WTEYLEATQT NAVPAKVFKM RLPHGFLPNM
301KLEVVDKRNP RLIRVATIVD VDDQRVKVHF DGWDHKYDYW VEADSPDIHP IGWCDVTGHP
361LEVPQRTNDL KILPGQAVCP TPGCRGIGHI RGPRYSGHHS AFGCPYSDMN LKKEATLHDR
421LREQTQANLE SDSSHSKSKS LCSLNFNGKH EKVNSQPRLV QQAKCLKIKG KEDIDLDNLF
481RVLVLHPRGL EYSVEQAQQV LHQSVSMSTV SAHPFRDLPL GREQHCKLLP GVADIRASQV
541ARWTVDEVAE FVQSLLGCEE HAKCFKKEQI DGKAFLLLTQ TDIVKVMKIK LGPALKIYNS
601ILMFRHSQEL PEEDIASGQE VRG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.