LSBio
L3MBTL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

L3MBTL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all L3MBTL3 antibodies →
$504.00each
Catalog Number: LS-G121335-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human L3MBTL3 (NM_001007102), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
L(3)mbt-like 3 (Drosophila) | L(3)mbt-like protein 3 | MBT1 | KIAA1798 | L3MBTL3 | RP11-73O6.1 | MBT-1 | H-l(3)mbt-like protein 3
Origin Species
Human
NCBI GenBank
NM_032438
SwissProt
Q96JM7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTESASSTSG QEFDVFSVMD WKDGVGTLPG SDLKFRVNEF GALEVITDEN EMENVKKATA
61TTTWMVPTAQ EVFSEKTGMP FRLKDPVKVE GLQFCENCCQ YGNVDECLSG GNYCSQNCAR
121HIKDKDQKEE RDVEEDNEEE DPKCSRKKKP KLSLKADNKE DGEERDDEME NKQDVRILRG
181SQRARRKRRG DSAVLKQGLP PKGKKAWCWA SYLEEEKAVA VPAKLFKEHQ SFPYNKNGFK
241VGMKLEGVDP EHQSVYCVLT VAEVCGYRIK LHFDGYSDCY DFWVNADALD IHPVGWCEKT
301GHKLHPPKGY KEEEFNWQTY LKTCKAQAAP KSLFENQNIT VIPSGFRVGM KLEAVDKKNP
361SFICVATVTD MVDNRFLVHF DNWDESYDYW CEASSPHIHP VGWCKEHRRT LITPPGYPNV
421KHFSWDKYLE ETNSLPAPAR AFKVKPPHGF QKKMKLEVVD KRNPMFIRVA TVADTDDHRV
481KVHFDGWNNC YDYWIDADSP DIHPVGWCSK TGHPLQPPLS PLELMEASEH GGCSTPGCKG
541IGHFKRARHL GPHSAANCPY SEINLNKDRI FPDRLSGEMP PASPSFPRNK RTDANESSSS
601PEIRDQHADD VKEDFEERTE SEMRTSHEAR GAREEPTVQQ AQRRSAVFLS FKSPIPCLPL
661RWEQQSKLLP TVAGIPASKV SKWSTDEVSE FIQSLPGCEE HGKVFKDEQI DGEAFLLMTQ
721TDIVKIMSIK LGPALKIFNS ILMFKAAEKN SHNEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

755 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.