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KY Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KY Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KY antibodies →
$725.00each
Catalog Number: LS-G104668-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KY (NM_178554), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KY | Kyphoscoliosis peptidase
Origin Species
Human
SwissProt
Q8NBH2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELKKDINAV SIDMLLIVHS EKRRAAQGTL SDQQANPSSL LQRGGGFQGV GNGVRRWQKL
61EGNDFHENLV EKQHPQQPQV ITSYNSQGTQ LTVEVHPRDA MPQLLKKFSL AKRLQGDKNG
121NTRPRQPGGK DAHAYPWDRS SLKSMSLDLQ QFEKLDIYAS QVTAKSGLDE LVSDLLQEAH
181TDLERVRAIW IWICHHIEYD IAAAQEKDRQ AFKPTDILRT QKTNCDGYAG LFERMCRLAG
241VQCMTVPGYS KGFGYQTGQS FSGEFDHAWN AVYLEGRWHL VDSTWGSGLV DTITSKFTFL
301YNEFYFLTHP ALFIEDHFPD NKNWQLLKPP QSLRQFENNM YHKSEFYNKG MLSAHPETSM
361IRTVNGKATV TIESCAPTLF MFMLNGKQEH GLLSLRKNGM KLEVYPPTMG THKLQIFAKG
421NSDIYSSVLE YTLKCNYVDM GVQLPAELHQ PVGPSWFSEQ MGIMKPSHPD PIIHTSDGRC
481SISFSVEEGI NVLASLHGDD GPITEETQRR YIFQLHREKQ TELKVQLPHA GKFALKIFVK
541KRQEPGNYIF VFNYLVCCAN TKVNWPMFPE SFGNWGQDNE LLEPLSGVLP ANRNVPFKLK
601LHGIAKVLVK GQDTWPLTLN HEGYWEGSCS TAGCQEVYVM VLENANHNFY SYILKYKVNA
661Q
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

661 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.