Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KV6.4 / KCNG4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105445-100
Catalog Number: LS-G105445
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNG4 (NM_172347), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KCNG4 | KV6.4 | KV6.3
- Origin Species
- Human
- NCBI GenBank
- NM_133490
- SwissProt
- Q8TDN1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPMPSRDGGL HPRHHHYGSH SPWSQLLSSP METPSIKGLY YRRVRKVGAL DASPVDLKKE61ILINVGGRRY LLPWSTLDRF PLSRLSKLRL CRSYEEIVQL CDDYDEDSQE FFFDRSPSAF121GVIVSFLAAG KLVLLQEMCA LSFQEELAYW GIEEAHLERC CLRKLLRKLE ELEELAKLHR181EDVLRQQRET RRPASHSSRW GLCMNRLREM VENPQSGLPG KVFACLSILF VATTAVSLCV241STMPDLRAEE DQGECSRKCY YIFIVETICV AWFSLEFCLR FVQAQDKCQF FQGPLNIIDI301LAISPYYVSL AVSEEPPEDG KRPSGSSYLE KVGLVLRVLR ALRILYVMRL ARHSLGLQTL361GLTVRRCTRE FGLLLLFLAV AITLFSPLVY VAEKESGRVL EFTSIPASYW WAIISMTTVG421YGDMVPHSVP GQMVALSSIL SGILIMAFPA TSIFHTFSHS YLELKKEQEQ LQARLRHLQN481TGPASECELL DPHVASEHEL MNDVNDLILE GPALPIMHMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
519 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.