Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Kv10.1 / KCNG3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105444-100
Catalog Number: LS-G105444
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNG3 (NM_172344), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KCNG3 | Kv10.1 | KV6.3
- Origin Species
- Human
- NCBI GenBank
- NM_133329
- SwissProt
- Q8TAE7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTFGRSGAAS VVLNVGGARY SLSRELLKDF PLRRVSRLHG CRSERDVLEV CDDYDRERNE61YFFDRHSEAF GFILLYVRGH GKLRFAPRMC ELSFYNEMIY WGLEGAHLEY CCQRRLDDRM121SDTYTFYSAD EPGVLGRDEA RPGGAEAAPS RRWLERMRRT FEEPTSSLAA QILASVSVVF181VIVSMVVLCA STLPDWRNAA ADNRSLDDRS RIIEAICIGW FTAECIVRFI VSKNKCEFVK241RPLNIIDLLA ITPYYISVLM TVFTGENSQL QRAGVTLRVL RMMRIFWVIK LARHFIGLQT301LGLTLKRCYR EMVMLLVFIC VAMAIFSALS QLLEHGLDLE TSNKDFTSIP AACWWVIISM361TTVGYGDMYP ITVPGRILGG VCVVSGIVLL ALPITFIYHS FVQCYHELKF RSARYSRSLS421TEFLNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
425 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.