Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KTN1 / Kinectin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104079-100
Catalog Number: LS-G104079
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kinectin 1(KTN1) (NM_182926), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CG-1 antigen | CG1 | Kinesin receptor | Kinectin | KNT | KIAA0004 | Kinectin 1 (kinesin receptor) | MU-RMS-40.19 | KTN1
- Origin Species
- Human
- NCBI GenBank
- NM_182926
- SwissProt
- Q86UP2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 156.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEFYESAYFI VLIPSIVITV IFLFFWLFMK ETLYDEVLAK QKREQKLIPT KTDKKKAEKK61KNKKKEIQNG NLHESDSESV PRDFKLSDAL AVEDDQVAPV PLNVVETSSS VRERKKKEKK121QKPVLEEQVI KESDASKIPG KKVEPVPVTK QPTPPSEAAA SKKKPGQKKS KNGSDDQDKK181VETLMVPSKR QEALPLHQET KQESGSGKKK ASSKKQKTEN VFVDEPLIHA TTYIPLMDNA241DSSPVVDKRE VIDLLKPDQV EGIQKSGTKK LKTETDKENA EVKFKDFLLS LKTMMFSEDE301ALCVVDLLKE KSGVIQDALK KSSKGELTTL IHQLQEKDKL LAAVKEDAAA TKDRCKQLTQ361EMMTEKERSN VVITRMKDRI GTLEKEHNVF QNKIHVSYQE TQQMQMKFQQ VREQMEAEIA421HLKQENGILR DAVSNTTNQL ESKQSAELNK LRQDYARLVN ELTEKTGKLQ QEEVQKKNAE481QAATQLKVQL QEAERRWEEV QSYIRKRTAE HEAAQQDLQS KFVAKENEVQ SLHSKLTDTL541VSKQQLEQRL MQLMESEQKR VNKEESLQMQ VQDILEQNEA LKAQIQQFHS QIAAQTSASV601LAEELHKVIA EKDKQIKQTE DSLASERDRL TSKEEELKDI QNMNFLLKAE VQKLQALANE661QAAAAHELEK MQQSVYVKDD KIRLLEEQLQ HEISNKMEEF KILNDQNKAL KSEVQKLQTL721VSEQPNKDVV EQMEKCIQEK DEKLKTVEEL LETGLIQVAT KEEELNAIRT ENSSLTKEVQ781DLKAKQNDQV SFASLVEELK KVIHEKDGKI KSVEELLEAE LLKVANKEKT VQDLKQEIKA841LKEEIGNVQL EKAQQLSITS KVQELQNLLK GKEEQMNTMK AVLEEKEKDL ANTGKWLQDL901QEENESLKAH VQEVAQHNLK EASSASQFEE LEIVLKEKEN ELKRLEAMLK ERESDLSSKT961QLLQDVQDEN KLFKSQIEQL KQQNYQQASS FPPHEELLKV ISEREKEISG LWNELDSLKD1021AVEHQRKKNN DLREKNWEAM EALASTEKML QDKVNKTSKE RQQQVEAVEL EAKEVLKKLF1081PKVSVPSNLS YGEWLHGFEK KAKECMAGTS GSEEVKVLEH KLKEADEMHT LLQLECEKYK1141SVLAETEGIL QKLQRSVEQE ENKWKVKVDE SHKTIKQMQS SFTSSEQELE RLRSENKDIE1201NLRREREHLE MELEKAEMER STYVTEVREL KDLLTELQKK LDDSYSEAVR QNEELNLLKA1261QLNETLTKLR TEQNERQKVA GDLHKAQQSL ELIQSKIVKA AGDTTVIENS DVSPETESSE1321KETMSVSLNQ TVTQLQQLLQ AVNQQLTKEK EHYQVLETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1357 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.