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KSR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KSR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KSR2 antibodies →
$725.00each
Catalog Number: LS-G105235-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KSR2 (NM_173598), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kinase suppressor of ras 2 | HKSR2 | KSR2
Origin Species
Human
NCBI GenBank
SK605D
SwissProt
Q6VAB6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIDLSISNLE GLRTKCATSN DLTQKEIRTL ESKLVKYFSR QLSCKKKVAL QERNAELDGF
61PQLRHWFRIV DVRKEVLEEI SPGQLSLEDL LEMTDEQVCE TVEKYGANRE ECARLNASLS
121CLRNVHMSGG NLSKQDWTIQ WPTTETGKEN NPVCPPEPTP WIRTHLSQSP RVPSKCVQHY
181CHTSPTPGAP VYTHVDRLTV DAYPGLCPPP PLESGHRSLP PSPRQRHAVR TPPRTPNIVT
241TVTPPGTPPM RKKNKLKPPG TPPPSSRKLI HLIPGFTALH RSKSHEFQLG HRVDEAHTPK
301AKKKSKPLNL KIHSSVGSCE NIPSQQRSPL LSERSLRSFF VGHAPFLPST PPVHTEANFS
361ANTLSVPRWS PQIPRRDLGN SIKHRFSTKY WMSQTCTVCG KGMLFGLKCK NCKLKCHNKC
421TKEAPPCHLL IIHRGDPARL VRTESVPCDI NNPLRKPPRY SDLHISQTLP KTNKINKDHI
481PVPYQPDSSS NPSSTTSSTP SSPAPPLPPS ATPPSPLHPS PQCTRQQKNF NLPASHYYKY
541KQQFIFPDVV PVPETPTRAP QVILHPVTSN PILEGNPLLQ IEVEPTSENE EVHDEAEESE
601DDFEEMNLSL LSARSFPRKA SQTSIFLQEW DIPFEQLEIG ELIGKGRFGQ VYHGRWHGEV
661AIRLIDIERD NEDQLKAFKR EVMAYRQTRH ENVVLFMGAC MSPPHLAIIT SLCKGRTLYS
721VVRDAKIVLD VNKTRQIAQE IVKGMGYLHA KGILHKDLKS KNVFYDNGKV VITDFGLFSI
781SGVLQAGRRE DKLRIQNGWL CHLAPEIIRQ LSPDTEEDKL PFSKHSDVFA LGTIWYELHA
841REWPFKTQPA EAIIWQMGTG MKPNLSQIGM GKEISDILLF CWAFEQEERP TFTKLMDMLE
901KLPKRNRRLS HPGHFWKSAE L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

921 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.