Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT83 / Keratin 83 / KRTHB3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118089-100
Catalog Number: LS-G118089
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRTHB3(KRT83) (NM_002282), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hard keratin | type II | 3 | HB3 | Hair keratin K2.10 | K83 | Keratin 83 | Keratin | hair | basic | type II cuticular Hb3 | HHb3 | Keratin-83 | KRT83 | KRTHB3 | Type-II keratin Kb23 | Hb-3 | Type II hair keratin Hb3
- Origin Species
- Human
- NCBI GenBank
- X99141
- SwissProt
- P78385
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTCGFNSIGC GFRPGNFSCV SACGPRPSRC CITAAPYRGI SCYRGLTGGF GSHSVCGGFR61AGSCGRSFGY RSGGVCGPSP PCITTVSVNE SLLTPLNLEI DPNAQCVKQE EKEQIKSLNS121RFAAFIDKVR FLEQQNKLLE TKLQFYQNCE CCQSNLEPLF AGYIETLRRE AECVEADSGR181LASELNHVQE VLEGYKKKYE EEVALRATAE NEFVALKKDV DCAYLRKSDL EANVEALIQE241IDFLRRLYEE EIRILQSHIS DTSVVVKLDN SRDLNMDCMV AEIKAQYDDI ATRSRAEAES301WYRSKCEEMK ATVIRHGETL RRTKEEINEL NRMIQRLTAE VENAKCQNSK LEAAVAQSEQ361QGEAALSDAR CKLAELEGAL QKAKQDMACL IREYQEVMNS KLGLDIEIAT YRRLLEGEEQ421RLCEGVEAVN VCVSSSRGGV VCGDLCVSGS RPVTGSVCSA PCNGNLVVST GLCKPCGQLN481TTCGGGSCGQ GRYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
493 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.