Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT80 / Keratin 80 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119749-100
Catalog Number: LS-G119749
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Keratin 80(KRT80) (NM_001081492), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cytokeratin-80 | Keratin 80 | K80 | KB20 | Keratin-80 | Type II keratin | Type-II keratin Kb20 | CK-80 | Keratin b20 | KRT80
- Origin Species
- Human
- NCBI GenBank
- NM_001081492
- SwissProt
- Q6KB66
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MACRSCVVGF SSLSSCEVTP VGSPRPGTSG WDSCRAPGPG FSSRSLTGCW SAGTISKVTV61NPGLLVPLDV KLDPAVQQLK NQEKEEMKAL NDKFASLIGK VQALEQRNQL LETRWSFLQG121QDSAIFDLGH LYEEYQGRLQ EELRKVSQER GQLEANLLQV LEKVEEFRIR YEDEISKRTD181MEFTFVQLKK DLDAECLHRT ELETKLKSLE SFVELMKTIY EQELKDLAAQ VKDVSVTVGM241DSRCHIDLSG IVEEVKAQYD AVAARSLEEA EAYSRSQLEE QAARSAEYGS SLQSSRSEIA301DLNVRIQKLR SQILSVKSHC LKLEENIKTA EEQGELAFQD AKTKLAQLEA ALQQAKQDMA361RQLRKYQELM NVKLALDIEI ATYRKLVEGE EGRMDSPSAT VVSAVQSRCK TAPSLPYPLC421SLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
422 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.