Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT74 / Keratin 74 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105119-100
Catalog Number: LS-G105119
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT74 (NM_175053), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK-74 | Cytokeratin-74 | KB37 | Keratin 74 | K5C | K6IRS4 | K74 | KRT6IRS4 | Keratin 6 irs4 | Keratin-5c | KRT5C | KRT74 | HTSS2 | Keratin 5c | Keratin-74 | Type-II keratin Kb37
- Origin Species
- Human
- SwissProt
- Q7RTS7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRQLNIKSS GDKGNFSVHS AVVPRKAVGS LASYCAAGRG AGAGFGSRSL YSLGGNRRIS61FNVAGGGVRA GGYGFRPGSG YGGGRASGFA GSMFGSVALG PACLSVCPPG GIHQVTVNKS121LLAPLNVELD PEIQKVRAQE REQIKVLNDK FASFIDKVRF LEQQNQVLET KWELLQQLDL181NNCKKNLEPI LEGYISNLRK QLETLSGDRV RLDSELRSMR DLVEDYKKRY EVEINRRTTA241ENEFVVLKKD ADAAYAVKVE LQAKVDSLDK EIKFLKCLYD AEIAQIQTHA SETSVILSMD301NNRDLDLDSI IAEVRMHYEE IALKSKAEAE ALYQTKIQEL QLAASRHGDD LKHTRSEMVE361LNRLIQRIRC EIGNVKKQRA SLETAIADAE QRGDNALKDA QAKLDELEGA LHQAKEELAR421MLREYQELMS LKLALDMEIA TYRKLLEGEE CRMSGENPSS VSISVISSSS YSYHHPSSAG481VDLGASAVAG SSGSTQSGQT KTTEARGGDL KDTQGKSTPA SIPARKATRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
529 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.