Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT73 / Keratin 73 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105049-100
Catalog Number: LS-G105049
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT73 (NM_175068), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cytokeratin-73 | CK-73 | IRT6IRS3 | Keratin 6 irs3 | Keratin-73 | K6IRS3 | KRT6IRS3 | KB36 | KRT73 | Type-II keratin Kb36 | K73 | Keratin 73
- Origin Species
- Human
- SwissProt
- Q86Y46
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRQFTYKSG AAAKGGFSGC SAVLSGGSSS SYRAGGKGLS GGFSSRSLYS LGGARSISFN61VASGSGWAGG YGFGRGRASG FAGSMFGSVA LGSVCPSLCP PGGIHQVTIN KSLLAPLNVE121LDPEIQKVRA QEREQIKVLN NKFASFIDKV RFLEQQNQVL ETKWELLQQL DLNNCKNNLE181PILEGYISNL RKQLETLSGD RVRLDSELRS VREVVEDYKK RYEEEINKRT TAENEFVVLK241KDVDAAYTSK VELQAKVDAL DGEIKFFKCL YEGETAQIQS HISDTSIILS MDNNRNLDLD301SIIAEVRAQY EEIARKSKAE AEALYQTKFQ ELQLAAGRHG DDLKHTKNEI SELTRLIQRL361RSEIESVKKQ CANLETAIAD AEQRGDCALK DARAKLDELE GALQQAKEEL ARMLREYQEL421LSVKLSLDIE IATYRKLLEG EECRMSGEYT NSVSISVINS SMAGMAGTGA GFGFSNAGTY481GYWPSSVSGG YSMLPGGCVT GSGNCSPRGE ARTRLGSASE FRDSQGKTLA LSSPTKKTMRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
540 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.