Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT72 / Keratin 72 / K6irs Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128168-100
Catalog Number: LS-G128168
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT72 (NM_001146226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK-72 | K6irs | K72 | Keratin protein K6irs | K6IRS2 | KRT6IRS2 | KB35 | Keratin 72 | KRT72 | Type-II keratin Kb35 | Cytokeratin-72 | Keratin-72 | KRT6
- Origin Species
- Human
- NCBI GenBank
- NM_080747
- SwissProt
- Q14CN4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRQLTHFPR GERLGFSGCS AVLSGGIGSS SASFRARVKG SASFGSKSLS CLGGSRSLAL61SAAARRGGGR LGGFVGTAFG SAGLGPKCPS VCPPGGIPQV TVNKSLLAPL NVEMDPEIQR121VRAQEREQIK ALNNKFASFI DKVRFLEQQN QVLETKWNLL QQLDLNNCRK NLEPIYEGYI181SNLQKQLEML SGDGVRLDSE LRNMQDLVED YKKRYEVEIN RRTAAENEFV VLKKDVDAAY241MNKVELQAKV DSLTDEIKFF KCLYEGEITQ IQSHISDTSI VLSMDNNRDL DLDSIIAEVR301AQYEEIALKS KAEAETLYQT KCADLETAIA DAEQRGDCAL KDARAKLDEL EGALHQAKEE361LARMLREYQE LVSLKLALDM EIATYRKLLE SEECRMSGEY PNSVSISVIS STNAGAGGAG421FSMGFGASSS YSYKTAAADV KTKGSCGSEL KDPLAKTSGS SCATKKASRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
469 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.