Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT6C / CK6C / Cytokeratin 6C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105412-100
Catalog Number: LS-G105412
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cytokeratin 6C(KRT6C) (NM_173086), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cytokeratin-6E | Keratin-6C | K6E | KRT6C | KRT6E | Keratin 6 isoform k6e | Keratin 6E | Type-II keratin Kb12 | CK-6C | CK-6E | Cytokeratin-6C | K6C | Keratin 6C | Keratin K6h
- Origin Species
- Human
- NCBI GenBank
- NM_173086
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTSTTIRS HSSSRRGFSA NSARLPGVSR SGFSSISVSR SRGSGGLGGA CGGAGFGSRS61LYGLGGSKRI SIGGGSCAIS GGYGSRAGGS YGFGGAGSGF GFGGGAGIGF GLGGGAGLAG121GFGGPGFPVC PPGGIQEVTV NQSLLTPLNL QIDPAIQRVR AEEREQIKTL NNKFASFIDK181VQFLEQQNKV LDTKWTLLQE QGTKTVRQNL EPLFEQYINN LRRQLDSIVG ERGRLDSELR241NMQDLVEDLK NKYEDEINKR TAAENEFVTL KKDVDAAYMN KVELQAKADT LTDEINFLRA301LYDAELSQMQ THISDTSVVL SMDNNRNLDL DSIIAEVKAQ YEEIAQRSRA EAESWYQTKY361EELQVTAGRH GDDLRNTKQE IAEINRMIQR LRSEIDHVKK QCASLQAAIA DAEQRGEMAL421KDAKNKLEGL EDALQKAKQD LARLLKEYQE LMNVKLALDV EIATYRKLLE GEECRLNGEG481VGQVNVSVVQ STISSGYGGA SGVGSGLGLG GGSSYSYGSG LGIGGGFSSS SGRAIGGGLS541SVGGGSSTIK YTTTSSSSRK SYKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
564 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.