Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT6A / CK6A / Cytokeratin 6A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115895-100
Catalog Number: LS-G115895
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT6A (NM_005554), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Allergen Hom s 5 | CK6A | CK-6D | CK6C | Cytokeratin 6C | Cytokeratin-6A | Cytokeratin-6D | CK-6A | Cytokeratin 6A | K6D | Keratin 6 isoform k6d | Keratin 6D | Keratin-6A | KRT6A | Type-II keratin Kb6 | CK6D | Cytokeratin 6D | K6A | K6C | Keratin 6 isoform k6a | Keratin 6A | Keratin 6C | KRT6D
- Origin Species
- Human
- NCBI GenBank
- NM_005554
- SwissProt
- P02538
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTSTTIRS HSSSRRGFSA SSARLPGVSR SGFSSVSVSR SRGSGGLGGA CGGAGFGSRS61LYGLGGSKRI SIGGGSCAIS GGYGSRAGGS YGFGGAGSGF GFGGGAGIGF GLGGGAGLAG121GFGGPGFPVC PPGGIQEVTV NQSLLTPLNL QIDPTIQRVR AEEREQIKTL NNKFASFIDK181VRFLEQQNKV LETKWTLLQE QGTKTVRQNL EPLFEQYINN LRRQLDSIVG ERGRLDSELR241GMQDLVEDFK NKYEDEINKR TAAENEFVTL KKDVDAAYMN KVELQAKADT LTDEINFLRA301LYDAELSQMQ THISDTSVVL SMDNNRNLDL DSIIAEVKAQ YEEIAQRSRA EAESWYQTKY361EELQVTAGRH GDDLRNTKQE IAEINRMIQR LRSEIDHVKK QCANLQAAIA DAEQRGEMAL421KDAKNKLEGL EDALQKAKQD LARLLKEYQE LMNVKLALDV EIATYRKLLE GEECRLNGEG481VGQVNISVVQ STVSSGYGGA SGVGSGLGLG GGSSYSYGSG LGVGGGFSSS SGRAIGGGLS541SVGGGSSTIK YTTTSSSSRK SYKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
564 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.