Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT40 / Hair Keratin KA36 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104280-100
Catalog Number: LS-G104280
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human keratin 40(KRT40) (NM_182497), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSDCSSTHC SPESCGTASG CAPASSCSVE TACLPGTCAT SRCQTPSFLS RSRGLTGCLL61PCYFTGSCNS PCLVGNCAWC EDGVFTSNEK ETMQFLNDRL ASYLEKVRSL EETNAELESR121IQEQCEQDIP MVCPDYQRYF NTIEDLQQKI LCTKAENSRL AVQLDNCKLA TDDFKSKYES181ELSLRQLLEA DISSLHGILE ELTLCKSDLE AHVESLKEDL LCLKKNHEEE VNLLREQLGD241RLSVELDTAP TLDLNRVLDE MRCQCETVLA NNRREAEEWL AVQTEELNQQ QLSSAEQLQG301CQMEILELKR TASALEIELQ AQQSLTESLE CTVAETEAQY SSQLAQIQCL IDNLENQLAE361IRCDLERQNQ EYQVLLDVKA RLEGEINTYW GLLDSEDSRL SCSPCSTTCT SSNTCEPCSA421YVICTVENCC LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
431 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.