Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT39 / Cytokeratin 39 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102617-100
Catalog Number: LS-G102617
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Keratin 39(KRT39) (NM_213656), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cytokeratin-39 | Keratin 39 | K39 | KA35 | KRT39 | Type I hair keratin Ka35 | CK-39 | Keratin-39
- Origin Species
- Human
- NCBI GenBank
- NM_213656
- SwissProt
- Q6A163
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTKGCTTTN SPSTPCQNCS RITNVSTISS NNGCHPGGLT VNNCQPAGHV LRIPWDQGCQ61PTPRFCRKPI YLMNNFNARF SLDDCSWYGE GINSNEKETM QILNERLANY LQKVRMLERE121NAELESKIQE ESNKELPVLC PDYLSYYTTI EELQQKILCT KAENSRLVSQ IDNTKLTADD181LRAKYEAEVS LRQLVESDAN GLKQILNVLT LGKADLEAQV QSLKEELLCL KNNHKEEINS241LQCQLGERLD IEVTAAPSAD LNQVLQEMRC QYEPIMETNR KDVEQWFNTQ IEELNQQVVT301SSQQQQCCQK EIIELRRSVN TLEVELQAQH RMRDSQECIL TETEARYTAL LTQIQSLIDN361LEAQLAEIRC ALERQNQEYE ILLDVKSRLE CEITTYRSLL ESSDGKRPCY PRATKCEPSP421WTSCKSGAIE STAPACTSSS PCSLKEHCSA CGPLSRILVK ICTITKEIKD GKVISSYEHV481QPCFIIRPAK VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
491 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.