Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT38 / Keratin 38 / KRTHA8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115091-100
Catalog Number: LS-G115091
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Keratin 38(KRT38) (NM_006771), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hair keratin | type I Ha8 | Keratin | hair | acidic | 8 | Keratin 38 | type I cuticular Ha8 | KRT38 | HHa8 | K38 | Keratin-38 | HA8 | HKA8 | KRTHA8
- Origin Species
- Human
- NCBI GenBank
- NM_006771
- SwissProt
- O76015
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSSYSSSSC PLGCTMAPGA RNVSVSPIDI GCQPGAEANI APMCLLANVA HANRVRVGST61PLGRPSLCLP PTCHTACPLP GTCHIPGNIG ICGAYGENTL NGHEKETMQF LNDRLANYLE121KVRQLEQENA ELEATLLERS KCHESTVCPD YQSYFHTIEE LQQKILCSKA ENARLIVQID181NAKLAADDFR IKLESERSLR QLVEADKCGT QKLLDDATLA KADLEAQQES LKEEQLSLKS241NHEQEVKILR SQLGEKLRIE LDIEPTIDLN RVLGEMRAQY EAMLETNRQD VEQWFQAQSE301GISLQDMSCS EELQCCQSEI LELRCTVNAL EVERQAQHTL KDCLQNSLCE AEDRFGTELA361QMQSLISNVE EQLSEIRADL ERQNQEYQVL LDVKTRLENE IATYRNLLES EDCKLPCNPC421STPPSCVTAP CAPRPSCGPC TTCGPTCGAS TTGSRFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.