Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT37 / Keratin 37 / KRTHA7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117102-100
Catalog Number: LS-G117102
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT37(KRT37) (NM_003770), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HA7 | Keratin 37 | Keratin | type I cuticular Ha7 | KRTHA7 | HHA7 | HKA7 | Hair keratin | type I Ha7 | K37 | hair | acidic | 7 | Keratin-37 | KRT37
- Origin Species
- Human
- NCBI GenBank
- NM_003770
- SwissProt
- O76014
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSFYSTSSC PLGCTMAPGA RNVFVSPIDV GCQPVAEANA ASMCLLANVA HANRVRVGST61PLGRPSLCLP PTSHTACPLP GTCHIPGNIG ICGAYGKNTL NGHEKETMKF LNDRLANYLE121KVRQLEQENA ELETTLLERS KCHESTVCPD YQSYFRTIEE LQQKILCSKA ENARLIVQID181NAKLAADDFR IKLESERSLH QLVEADKCGT QKLLDDATLA KADLEAQQES LKEEQLSLKS241NHEQEVKILR SQLGEKFRIE LDIEPTIDLN RVLGEMRAQY EAMVETNHQD VEQWFQAQSE301GISLQAMSCS EELQCCQSEI LELRCTVNAL EVERQAQHTL KDCLQNSLCE AEDRYGTELA361QMQSLISNLE EQLSEIRADL ERQNQEYQVL LDVKARLENE IATYRNLLES EDCKLPCNPC421STPASCTSCP SCGPVTGGSP SGHGASMGRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
449 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.