Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT36 / Keratin 36 / KRTHA6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117103-100
Catalog Number: LS-G117103
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Keratin 36(KRT36) (NM_003771), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- K36 | Keratin | hair | acidic | 6 | Keratin-36 | type I cuticular Ha6 | KRTHA6 | KRT36 | Hair keratin | type I Ha6 | HKA6 | HA6 | HHa6 | Keratin 36
- Origin Species
- Human
- NCBI GenBank
- NM_003771
- SwissProt
- O76013
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATQTCTPTF STGSIKGLCG TAGGISRVSS IRSVGSCRVP SLAGAAGYIS SARSGLSGLG61SCLPGSYLSS ECHTSGFVGS GGWFCEGSFN GSEKETMQFL NDRLANYLEK VRQLERENAE121LESRIQEWYE FQIPYICPDY QSYFKTIEDF QQKILLTKSE NARLVLQIDN AKLAADDFRT181KYETELSLRQ LVEADINGLR RILDELTLCK ADLEAQVESL KEELMCLKKN HEEEVSVLRC241QLGDRLNVEV DAAPPVDLNK ILEDMRCQYE ALVENNRRDV EAWFNTQTEE LNQQVVSSSE301QLQCCQTEII ELRRTVNALE IELQAQHSMR NSLESTLAET EARYSSQLAQ MQCLISNVEA361QLSEIRCDLE RQNQEYQVLL DVKARLEGEI ATYRHLLEGE DCKLPPQPCA TACKPVIRVP421SVPPVPCVPS VPCTPAPQVG TQIRTITEEI RDGKVISSRE HVQSRPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
467 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.