Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT35 / Keratin 35 / KRTHA5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118087-100
Catalog Number: LS-G118087
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT35 (NM_002280), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HA5 | Hard keratin | type I | 5 | HHa5 | Keratin | type I cuticular Ha5 | KRTHA5 | Ha-5 | Hair keratin | type I Ha5 | HKA5 | Keratin 35 | K35 | hair | acidic | Keratin-35 | KRT35
- Origin Species
- Human
- NCBI GenBank
- NM_002280
- SwissProt
- Q92764
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASKCLKAGF SSGSLKSPGG ASGGSTRVSA MYSSSSCKLP SLSPVARSFS ACSVGLGRSS61YRATSCLPAL CLPAGGFATS YSGGGGWFGE GILTGNEKET MQSLNDRLAG YLEKVRQLEQ121ENASLESRIR EWCEQQVPYM CPDYQSYFRT IEELQKKTLC SKAENARLVV EIDNAKLAAD181DFRTKYETEV SLRQLVESDI NGLRRILDDL TLCKSDLEAQ VESLKEELLC LKKNHEEEVN241SLRCQLGDRL NVEVDAAPPV DLNRVLEEMR CQYETLVENN RRDAEDWLDT QSEELNQQVV301SSSEQLQSCQ AEIIELRRTV NALEIELQAQ HSMRDALEST LAETEARYSS QLAQMQCMIT361NVEAQLAEIR ADLERQNQEY QVLLDVRARL ECEINTYRGL LESEDSKLPC NPCAPDYSPS421KSCLPCLPAA SCGPSAARTN CSPRPICVPC PGGRFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
455 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.