Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT33B / Keratin 33B / KRTHA3B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118086-100
Catalog Number: LS-G118086
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRTHA3B(KRT33B) (NM_002279), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hard keratin | type I | 3II | HA3II | Hair keratin | type I Ha3-II | K33B | Keratin | hair | acidic | 3B | KRT33B | KRTHA3A | KRTHA3B | Ha-3II | HKA3B | Keratin 33B | Type I hair keratin 3B | HHa3-II | Keratin-33B
- Origin Species
- Human
- NCBI GenBank
- NM_002279
- SwissProt
- Q14525
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPYNFCLPSL SCRTSCSSRP CVPPSCHGYT LPGACNIPAN VSNCNWFCEG SFNGSEKETM61QFLNDRLASY LEKVRQLERD NAELENLIRE RSQQQEPLLC PSYQSYFKTI EELQQKILCS121KSENARLVVQ IDNAKLAADD FRTKYQTEQS LRQLVESDIN SLRRILDELT LCRSDLEAQM181ESLKEELLSL KQNHEQEVNT LRCQLGDRLN VEVDAAPAVD LNQVLNETRN QYEALVETNR241REVEQWFATQ TEELNKQVVS SSEQLQSYQA EIIELRRTVN ALEIELQAQH NLRYSLENTL301TESEARYSSQ LSQVQSLITN VESQLAEIRS DLERQNQEYQ VLLDVRARLE CEINTYRSLL361ESEDCKLPSN PCATTNACEK PIGSCVTNPC GPRSRCGPCN TFGYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
404 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.