Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT33A / Keratin 33A / KRTHA3A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116842-100
Catalog Number: LS-G116842
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT33A (NM_004138), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hard keratin | type I | 3I | K33A | Keratin | hair | acidic | 3A | Krt1-3 | KRTHA3A | KRT33A | Ha-3I | HHa3-I | HKA3A | Keratin 33A | Keratin-33A | HA3I | Hair keratin | type I Ha3-I
- Origin Species
- Human
- NCBI GenBank
- NM_004138
- SwissProt
- O76009
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYSCGLPSL SCRTSCSSRP CVPPSCHGCT LPGACNIPAN VSNCNWFCEG SFNGSEKETM61QFLNDRLASY LEKVRQLERD NAELENLIRE RSQQQEPLVC ASYQSYFKTI EELQQKILCS121KSENARLVVQ IDNAKLASDD FRTKYETELS LRQLVESDIN GLRRILDELT LCRSDLEAQV181ESLKEELLCL KQNHEQEVNT LRCQLGDRLN VEVDAAPTVD LNQVLNETRS QYEALVETNR241REVEQWFATQ TEELNKQVVS SSEQLQSYQA EIIELRRTVN ALEIELQAQH NLRDSLENTL301TESEARYSSQ LSQVQRLITN VESQLAEIRS DLERQNQEYQ VLLDVRARLE CEINTYRSLL361ESEDCKLPSN PCATTNACDK STGPCISNPC GLRARCGPCN TFGYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
404 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.