Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT26 / Keratin 26 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104453-100
Catalog Number: LS-G104453
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT26 (NM_181539), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK-26 | CK26 | Cytokeratin-26 | Keratin 25B | Keratin 26 | K25IRS2 | K26 | Keratin-26 | KRT26 | K25B | Keratin-25B | KRT25B
- Origin Species
- Human
- SwissProt
- Q7Z3Y9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSFRLSGGSR RICSRTGSGR LSGGGTGFVA GNVCVGSGAR SSFSCTLEGI SSGGSFCNSG61GGLGSGACAG FLGNEHSLLS GNEKVTMQNL NDRLASYLDH VHALEEANAD LEQKIKGWYE121KCEPGSSREH DHDYSRYFSV IEDLKRQIIS ATICNASIVL QNDNARLTAD DFRLKYENEL181ALHHSVEADT SGLRRVLDEL TLCTTDLEIQ CETLSEELTY LKKSHEEEME VLQYTAGGNV241NVEMNATPGV DLTVLLNNMR AEYEDLAEQN RKDAEAWFNE RSATLQQQIS DHEGAATAAR301NELTELKRNL QTLEIELQSL MAVKHSYECS LAETEGNYCN QLQQIQDQIG VMEEQLQQIR361TETEGQKLEY EQLLDVKIFL EKEIDIYCNL LDGEERKSKS TCYKSKGYRP VNSGNQAKDS421TEETIVKTVV EELDQIGNLL SLRVHSVEEK SSKISNITVE QRVPSKAPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
468 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.