Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT24 / Keratin 24 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111491-100
Catalog Number: LS-G111491
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT24 (NM_019016), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK-24 | Keratin-24 | Keratin 24 | Cytokeratin-24 | K24 | KA24 | KRT24 | Type I keratin-24
- Origin Species
- Human
- NCBI GenBank
- NM_019016
- SwissProt
- Q2M2I5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSCSSRASSS RAGGSSSARV SAGGSSFSSG SRCGLGGSSA QGFRGGASSC SLSGGSSGAF61GGSFGGGFGS CSVGGGFGGA SGSGTGFGGG SSFGGVSGFG RGSGFCGSSR FSSGATGGFY121SYGGGMGGGV GDGGLFSGGE KQTMQNLNDR LANYLDKVRA LEEANTDLEN KIKEWYDKYG181PGSGDGGSGR DYSKYYSIIE DLRNQIIAAT VENAGIILHI DNARLAADDF RLKYENELCL241RQSVEADING LRKVLDDLTM TRSDLEMQIE SFTEELAYLR KNHEEEMKNM QGSSGGEVTV301EMNAAPGTDL TKLLNDMRAQ YEELAEQNRR EAEERFNKQS ASLQAQISTD AGAATSAKNE361ITELKRTLQA LEIELQSQLA MKSSLEGTLA DTEAGYVAQL SEIQTQISAL EEEICQIWGE421TKCQNAEYKQ LLDIKTRLEV EIETYRRLLD GEGGGSSFAE FGGRNSGSVN MGSRDLVSGD481SRSGSCSGQG RDSSKTRVTK TIVEELVDGK VVSSQVSSIS EVKVKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
525 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.