Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT222 / Keratin 222 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106322-100
Catalog Number: LS-G106322
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT222P(KRT222) (NM_152349), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Keratin 222 | Keratin-like protein KRT222 | KRT222 | KRT222P | KA21 | Keratin-222 | Keratin 222 pseudogene | Keratin-222 pseudogene
- Origin Species
- Human
- NCBI GenBank
- NM_152349
- SwissProt
- Q8N1A0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MELSQLLNEI RANYEKILTR NQIETVLSTR IQLEEDISKK MDKDEEALKA AQAELKEARR61QWHHLQVEIE SLHAVERGLE NSLHASEQHY QMQLQDLETV IEGLEKELQE VRRGIEKQLQ121EHEMLLNTKM RLEQEIATYR HLLEKEEIRY YGCIQGGKKD KKPTTSRVGF VLPSAIINEI181SFTTKVPQKY ENENVETVTK QAILNGSIVK ESTEAHGTIQ TEKVDEVIKE WEGSFFKDNP241RLRKKSVSLR FDLHLAATDE GCLETKQDNL PDIEVRLIMR RSCSIPSIKP PSTANSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
295 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.