Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT15 / CK15 / Cytokeratin 15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118082-100
Catalog Number: LS-G118082
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cytokeratin 15(KRT15) (NM_002275), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK15 | Cytokeratin 15 | CK-15 | K1CO | Keratin 15 | K15 | Keratin-15 | basic | KRT15 | KRTB | Cytokeratin-15 | beta | Type I cytoskeletal 15
- Origin Species
- Human
- NCBI GenBank
- NM_002275
- SwissProt
- P19012
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTTFLQTSS STFGGGSTRG GSLLAGGGGF GGGSLSGGGG SRSISASSAR FVSSGSGGGY61GGGMRVCGFG GGAGSVFGGG FGGGVGGGFG GGFGGGDGGL LSGNEKITMQ NLNDRLASYL121DKVRALEEAN ADLEVKIHDW YQKQTPTSPE CDYSQYFKTI EELRDKIMAT TIDNSRVILE181IDNARLAADD FRLKYENELA LRQGVEADIN GLRRVLDELT LARTDLEMQI EGLNEELAYL241KKNHEEEMKE FSSQLAGQVN VEMDAAPGVD LTRVLAEMRE QYEAMAEKNR RDVEAWFFSK301TEELNKEVAS NTEMIQTSKT EITDLRRTMQ ELEIELQSQL SMKAGLENSL AETECRYATQ361LQQIQGLIGG LEAQLSELRC EMEAQNQEYK MLLDIKTRLE QEIATYRSLL EGQDAKMAGI421GIREASSGGG GSSSNFHINV EESVDGQVVS SHKREITRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.