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KRI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KRI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110168-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRI1 (NM_023008), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KRI1 | KRI1 homolog (S. cerevisiae) | Protein KRI1 homolog
Origin Species
Human
NCBI GenBank
NM_023008
SwissProt
Q8N9T8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGHRTAMPEP RGSSQLRVNA AFAARYNRYR EREELQRLKD RYGDRDSSSD SSSESDSSDE
61RVEFDPQQER DFYKTLSLLK KKDPRIYQKD ATFYNRTASS SDSEEDPEAL EKQKKVRPMY
121LKDYERKVIL EKAGKYVDEE NSDGETSNHR LQETSSQSYV EEQKQLKESF RAFVEDSEDE
181DGAGEGGSSL LQKRAKTRQE KAQEEADYIE WLKGQKEIRN PDSLKELTHL KEYWNDPELD
241EGERFLRDYI LNKRYEEEEE EEEDEEEMEE EEGVHGPPVQ LAVDDSSDEG ELFLKKQEDF
301EQKYNFRFEE PDSASVKTYP RSIASSVRRK DERRKEKREE TRERKKREKA KKQEELKQLK
361NLKRKEILAK LEKLRKVTGN EMLGLEEGDL EDDFDPAQHD QLMQKCFGDE YYGAVEEEKP
421QFEEEEGLED DWNWDTWDGP EQEGDWSQQE LHCEDPNFNM DADYDPSQPR KKKREAPLTG
481KKKRKSPFAA AVGQEKPVFE PGDKTFEEYL DEYYRLDYED IIDDLPCRFK YRTVVPCDFG
541LSTEEILAAD DKELNRWCSL KKTCMYRSEQ EELRDKRAYS QKAQNSWKKR QVFKSLCREE
601AETPAEATGK PQRDEAGPQR QLPALDGSLM GPESPPAQEE EAPVSPHKKP APQKRRRAKK
661ARLLGPTVML GGCEFSRQRL QAFGLNPKRL HFRQLGRQRR KQQGPKNSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

709 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.