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KRBA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KRBA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102704-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRBA2 (NM_213597), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KRAB-A domain containing 2 | KRBA2 | KRAB A domain containing 2
Origin Species
Human
NCBI GenBank
NM_213597
SwissProt
Q6ZNG9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSFLVPSLV SSPVLLKLLF SPGPKTIWSL WQQPMLFQEA TAFENMTKDW NYLEGSQKDC
61YRDTMLDSYE NTVPQGSFLQ LSMMPQRAGN DPPGVSNASE MEMEISNMRE KFLMSVTKLV
121ESKSYNSKVF SKEKYFQTIK EVKEAKEKGK KSSRDYRRAA KYDVISVQGT EKLIEATHGE
181RDRIRYYVHK EELFDILHDT HLSIGHGGRT RMLKELQGKY GNVTKEVIVL YLTLCKQCHQ
241KNPVPKRGLA PKPMTFKDID STCQVEILDM QSSADGEFKF ILYYQDHSTK FIILRPLRTK
301QAHEVVSVLL DIFTILGTPS VLDSDSGVEF TNQVVHELNE LWPDLKIVSG KYHPGQSQGS
361LEGASRDVKN MISTWMQSNH SCHWAKGLRF MQMVRNQAFD VSLQQSPFEA MFGYKAKFGL
421YSSNLPRETV ATLQTEEELE IAEEQLENSL WIRQEERAEI GADRSDMDDD MDPTPEASEP
481STSQGTSGLL CW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

492 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.