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KRBA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KRBA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108757-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRBA1 (NM_032534), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KRAB-A domain containing 1 | KIAA1862 | KRAB A domain containing 1 | KRBA1 | Protein KRBA1
Origin Species
Human
NCBI GenBank
NM_032534
SwissProt
A5PL33

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRENYETLVS VGTAELLPLS AFLSPSEPGR AVGGGSHADE GQEPAGCGDP QGGQPRHSLH
61LTALVQLVKE IPEFLFGEVK GAMDSPESES RGASLDGERA SPEAAAAREP CPLRGLLSCL
121PDGPTSQPHL ATTPTDSSCS SGPTGDGVQG SPLPIKTADK PWPTRKEGPG ALGGEPSPPT
181HSPSRRKSHR GQERGTSEAG ISPGNSPLQG LINCLKEILV PGPRHPETSP SFLPPLPSLG
241TSRLTRADLG PGSPPWAVKT EAVSGDCPLQ GLLHCLKELP EAQDRHPSPS GVGNRRLQEN
301PGAWKRGSGG PGYLLTPPPH PDLGAGGLLS VKMENSWVQS PPGPASCQPG RQPLSPSATG
361DTRGVPQPSW GPEAQAASAS SSPLEALEAC LKGIPPNGSS PSQLPPTSCS QNPQPGDSRS
421QKPELQPHRS HSEEATREPV LPLGLQSCVR DGPSRPLAPR GTPTSFSSSS STDWDLDFGS
481PVGNQGQHPG KGSPPGSSPL QGLENCLKEI PVPVLRPAWP CSSAADRGPR RAEPRNWTAD
541KEGLRAEACE SARLGQGRGE APTRSLHLVS PQVFTSSCVP ACHQRGFKDP GATRPGVWRW
601LPEGSAPKPS PLHCLESALR GILPVRPLRF ACVGGPSPSP SPGSSSSFSG SEGEDPRPEP
661ELWKPLPQER DRLPSCKPPV PLSPCPGGTP AGSSGGSPGE DPRRTEPRYC SGLGAGTAQD
721PCPVSQLEKR PRVSEASRGL ELGHGRPRVA AKTHERLLPQ GPPELPSESP PPELPPPEAA
781PPVLPASSLQ PPCHCGKPLQ QELHSLGAAL AEKLDRLATA LAGLAQEVAT MRTQVNRLGR
841RPQGPGPMGQ ASWMWTLPRG PRWAHGPGHR HLPYWRQKGP TRPKPKILRG QGESCRAGDL
901QGLSRGTARR ARPLPPDAPP AEPPGLHCSS SQQLLSSTPS CHAAPPAHPL LAHTGGHQSP
961LPPLVPAALP LQGASPPAAS ADADVPTSGV APDGIPERPK EPSSLLGGVQ RALQEELWGG
1021EHRDPRWGAH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1030 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.