Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KPTN / Kaptin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114889-100
Catalog Number: LS-G114889
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kaptin(KPTN) (NM_007059), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 2E4 | Actin-associated protein 2E4 | Kaptin | Kaptin (actin binding protein) | KPTN | Kaptin (actin-binding protein)
- Origin Species
- Human
- NCBI GenBank
- NM_007059
- SwissProt
- Q9Y664
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMGEAAVAAG PCPLREDSFT RFSSQSNVYG LAGGAGGRGE LLAATLKGKV LGFRYQDLRQ61KIRPVAKELQ FNYIPVDAEI VSIDTFNKSP PKRGLVVGIT FIKDSGDKGS PFLNIYCDYE121PGSEYNLDSI AQSCLNLELQ FTPFQLCHAE VQVGDQLETV FLLSGNDPAI HLYKENEGLH181QFEEQPVENL FPELTNLTSS VLWLDVHNFP GTSRRLSALG CQSGYVRVAH VDQRSREVLQ241MWSVLQDGPI SRVIVFSLSA AKETKDRPLQ DEYSVLVASM LEPAVVYRDL LNRGLEDQLL301LPGSDQFDSV LCSLVTDVDL DGRPEVLVAT YGQELLCYKY RGPESGLPEA QHGFHLLWQR361SFSSPLLAMA HVDLTGDGLQ ELAVVSLKGV HILQHSLIQA SELVLTRLRH QVEQRRRRLQ421GLEDGAGAGP AENAASTRTR PLEQKLISEE DLAANDILDY KDDDDKV
467 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.