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KPNA7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KPNA7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126804-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KPNA7 (NM_001145715), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Importin subunit alpha-8 | Karyopherin subunit alpha-7 | KPNA7 | Karyopherin 7
Origin Species
Human
SwissProt
A9QM74

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTLDAPEER RRKFKYRGKD VSLRRQQRMA VSLELRKAKK DEQTLKRRNI TSFCPDTPSE
61KTAKGVAVSL TLGEIIKGVN SSDPVLCFQA TQTARKMLSQ EKNPPLKLVI EAGLIPRMVE
121FLKSSLYPCL QFEAAWALTN IASGTSEQTR AVVEGGAIQP LIELLSSSNV AVCEQAVWAL
181GNIAGDGPEF RDNVITSNAI PHLLALISPT LPITFLRNIT WTLSNLCRNK NPYPCDTAVK
241QILPALLHLL QHQDSEVLSD ACWALSYLTD GSNKRIGQVV NTGVLPRLVV LMTSSELNVL
301TPSLRTVGNI VTGTDEQTQM AIDAGMLNVL PQLLQHNKPS IQKEAAWALS NVAAGPCHHI
361QQLLAYDVLP PLVALLKNGE FKVQKEAVWM VANFATGATM DQLIQLVHSG VLEPLVNLLT
421APDVKIVLII LDVISCILQA AEKRSEKENL CLLIEELGGI DRIEALQLHE NRQIGQSALN
481IIEKHFGEEE DESQTLLSQV IDQDYEFIDY ECLAKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.