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KPNA5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KPNA5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G99707-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KPNA5 (NM_002269), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Importin subunit alpha-6 | Karyopherin subunit alpha-5 | IPOA6 | KPNA5 | Importin alpha 6 | SRP6
NCBI GenBank
NM_002269
SwissProt
O15131

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDAMASPGKD NYRMKSYKNK ALNPQEMRRR REEEGIQLRK QKREEQLFKR RNVYLPRNDE
61SMLESPIQDP DISSTVPIPE EEVVTTDMVQ MIFSNNADQQ LTATQKFRKL LSKEPNPPID
121QVIQKPGVVQ RFVKFLERNE NCTLQFEAAW ALTNIASGTF LHTKVVIETG AVPIFIKLLN
181SEHEDVQEQA VWALGNIAGD NAECRDFVLN CEILPPLLEL LTNSNRLTTT RNAVWALSNL
241CRGKNPPPNF SKVSPCLNVL SRLLFSSDPD VLADVCWALS YLSDGPNDKI QAVIDSGVCR
301RLVELLMHND YKVVSPALRA VGNIVTGDDI QTQVILNCSA LPCLLHLLSS PKESIRKEAC
361WTVSNITAGN RAQIQAVIDA NIFPVLIEIL QKAEFRTRKE AAWAITNATS GGTPEQIRYL
421VALGCIKPLC DLLTVMDSKI VQVALNGLEN ILRLGEQESK QNGIGINPYC ALIEEAYGLD
481KIEFLQSHEN QEIYQKAFDL IEHYFGVEED DPSIVPQVDE NQQQFIFQQQ EAPMDGFQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

539 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.