Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KPNA3 / Importin Alpha 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99706-100
Catalog Number: LS-G99706
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KPNA3 (NM_002267), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Importin alpha-3 | Importin alpha Q2 | Karyopherin subunit alpha-3 | Importin-alpha-Q2 | IPOA4 | Qip2 | SRP1 | HSRP1 | Importin alpha 4 | Importin subunit alpha-3 | KPNA3 | SRP1-gamma | SRP1gamma | SRP4
- NCBI GenBank
- NM_002267
- SwissProt
- O00505
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAENPSLENH RIKSFKNKGR DVETMRRHRN EVTVELRKNK RDEHLLKKRN VPQEESLEDS61DVDADFKAQN VTLEAILQNA TSDNPVVQLS AVQAARKLLS SDRNPPIDDL IKSGILPILV121KCLERDDNPS LQFEAAWALT NIASGTSAQT QDIVQSNAVP LFLRLLRSPH QNVCEQAVWA181LGNIIGDGPQ CRDYVISLGV VKPLLSFISP SIPITFLRNV TWVIVNLCRN KDPPPPMETV241QEILPALCVL IYHTDINILV DTVWALSYLT DGGNEQIQMV IDSGVVPFLV PLLSHQEVKV301QTAALRAVGN IVTGTDEQTQ VVLNCDVLSH FQNLLSHPKE KINKEAVWFL SNITAGNQQQ361VQAVIDAGLI PMIIHQLAKG DFGTQKEAAW AISNLTISGR KDQVEYLVQQ DVIPPFCNLL421SVKDSQVVQV VLDGLKNILI MAGDEASTIA EIIEECGGLE KIEVLQQHEN EDIYKLAFEI481IDQYFSGDDI DEDPCLIPEA TQGGTYNFDP TANLQTKEFN FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
521 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.