Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KPI-2 / LMTK2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113591-100
Catalog Number: LS-G113591
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KPI2(LMTK2) (NM_014916), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AATYK2 | Brain-enriched kinase | Cprk | Kinase phosphatase inhibitor 2 | Kinase/phosphatase/inhibitor 2 | KPI2 | LMR2 | LMTK2 | HBREK | BREK | CDK5/p35-regulated kinase | KIAA1079 | KPI-2 | Lemur tyrosine kinase 2
- Origin Species
- Human
- NCBI GenBank
- NM_014916
- SwissProt
- Q8IWU2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 164.92 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGPPALRRR LLLLLLVLLI AGSAGAAPLP QTGAGEAPPA AEVSSSFVIL CVCSLIILIV61LIANCVSCCK DPEIDFKEFE DNFDDEIDFT PPAEDTPSVQ SPAEVFTLSV PNISLPAPSQ121FQPSVEGLKS QVARHSLNYI QEIGNGWFGK VLLGEIYTGT SVARVIVKEL KASANPKEQD181TFLKNGEPYY ILQHPNILQC VGQCVEAIPY LLVFEFCDLG DLKAYLRSEQ EHMRGDSQTM241LLQRMACEVA AGLAAMHKLH FLHSDLALRN CFLTSDLNVK VGDYGIGFSR YKEDYIETDD301KKVFPLRWTA PELVTSFQDR LLTADQTKYS NIWSLGVTLW ELFDNAAQPY SNLSNLDVLN361QVIRERDTKL PKPQLEQPYS DRWYEVLQFC WLSPEKRPAA EDVHRLLTYL RLQSQRDSEV421DFEQQWNALK PNTNSRDSSN NAAFPILDHF ARDRLGREME EVLTVTETSQ GLSFEYVWEA481AKHDHFDERS RGHLDEGLSY TSIFYPVEVF ESSLSDPGPG KQDDSGQDVP LRVPGVVPVF541DAHNLSVGSD YYIQLEEKSG SNLELDYPPA LLTTDMDNPE RTGPELSQLT ALRSVELEES601STDEDFFQSS TDPKDSSLPG DLHVTSGPES PFNNIFNDVD KSEDLPSHQK IFDLMELNGV661QADFKPATLS SSLDNPKESV ITGHFEKEKP RKIFDSEPLC LSDNLMHQDN FDPLNVQELS721ENFLFLQEKN LLKGSLSSKE HINDLQTELK NAGFTEAMLE TSCRNSLDTE LQFAENKPGM781SLLQENVSTK GDDTDVMLTG DTLSTSLQSS PEVQVPPTSF ETEETPRRVP PDSLPTQGET841QPTCLDVIVP EDCLHQDISP DAVTVPVEIL STDARTHSLD NRSQDSPGES EETLRLTESD901SVLADDILAS RVSVGSSLPE LGQELHNKPF SEDHHSHRRL EKNLEAVETL NQLNSKDAAK961EAGLVSALSS DSTSQDSLLE DSLSAPFPAS EPSLETPDSL ESVDVHEALL DSLGSHTPQK1021LVPPDKPADS GYETENLESP EWTLHPAPEG TADSEPATTG DGGHSGLPPN PVIVISDAGD1081GHRGTEVTPE TFTAGSQGSY RDSAYFSDND SEPEKRSEEV PGTSPSALVL VQEQPLPEPV1141LPEQSPAAQD SCLEARKSQP DESCLSALHN SSDLELRATP EPAQTGVPQQ VHPTEDEASS1201PWSVLNAELS SGDDFETQDD RPCTLASTGT NTNELLAYTN SALDKSLSSH SEGPKLKEPD1261IEGKYLGKLG VSGMLDLSED GMDADEEDEN SDDSDEDLRA FNLHSLSSES EDETEHPVPI1321ILSNEDGRHL RSLLKPTAAN APDPLPEDWK KEKKAVTFFD DVTVYLFDQE TPTKELGPCG1381GEACGPDLSG PAPASGSPYL SRCINSESST DEEGGGFEWD DDFSPDPFMS KTTSNLLSSK1441PSLQTSKYFS PPPPARSTEQ SWPHSAPYSR FSISPANIAS FSLTHLTDSD IEQGGSSEDG1501EKDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1503 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.