Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KNOP1 / C16orf88 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121475-100
Catalog Number: LS-G121475
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C16orf88(KNOP1) (NM_001012991), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 101F10.1 | C16orf88 | FAM191A | TSG118 | Protein C16orf88
- Origin Species
- Human
- NCBI GenBank
- NM_001012991
- SwissProt
- Q1ED39
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MITKTHKVDL GLPEKKKKKK VVKEPETRYS VLNNDDYFAD VSPLRATSPS KSVAHGQAPE61MPLVKKKKKK KKGVSTLCEE HVEPETTLPA RRTEKSPSLR KQVFGHLEFL SGEKKNKKSP121LAMSHASGVK TSPDPRQGEE ETRVGKKLKK HKKEKKGAQD PTAFSVQDPW FCEAREARDV181GDTCSVGKKD EEQAALGQKR KRKSPREHNG KVKKKKKIHQ EGDALPGHSK PSRSMESSPR241KGSKKKPVKV EAPEYIPISD DPKASAKKKM KSKKKVEQPV IEEPALKRKK KKKRKESGVA301GDPWKEETDT DLEVVLEKKG NMDEAHIDQV RRKALQEEID RESGKTEASE TRKWTGTQFG361QWDTAGFENE DQKLKFLRLM GGFKNLSPSF SRPASTIARP NMALGKKAAD SLQQNLQRDY421DRAMSWKYSR GAGLGFSTAP NKIFYIDRNA SKSVKLEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
458 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.