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KNDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KNDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106099-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KNDC1 (NM_152643), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BB439H18.3 | KIAA1768 | RP13-439H18.3 | Protein very KIND | RasGEF domain family | member 2 | RASGEF2 | VKIND | C10orf23 | Cerebral protein 9 | KNDC1
Origin Species
Human
SwissProt
Q76NI1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
191.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQAMDPAAAD LYEEDGKDLD FYDFEPLPTL PEDEENVSLA DILSLRDRGL SEQEAWAVCL
61ECSLSMRSVA HAAIFQSLCI TPDTLAFNTS GNVCFMEQLS DDPEGAFVPP EFDVTGNTFE
121AHIYSLGATL KAALEYVAEP TLEPRLSQDL EALLSRMQAE DPGDRPDLES IIALCEEKLQ
181LTSSCRVCRS LSAVGRRVLS IESFGALQDV SESSWRERPA PGNAGPRRPP GDPSTDPEVL
241PTPEGPESET SRGPRASPTK ALLSTPVRNG ESHSREGLAG LVLDAERTLG ELDRDALRRS
301RLRKVQTFPR LLSDSPEATL CLPLTRGKSQ LPISELFSPD PRKAFLDRKN GLSSFQAQPK
361CRLWPEQEPE HQLGRVPCAG RSTDRGPGVP GSPGQPETSH PSQGPAEAPA DPRDASGEAQ
421TPRDDERIPE GARQLESAAA EQWVSLQDLL SQLGRPFREY ELWALCLACL RALQTRPEHP
481AYLCLDSVLV AEDGAVLFQP PPANGSYDSF FLAPELAEER LVTEKASVYC VAAVLWTAAK
541FSVPRNHKLA LPRRLKTLLL DMARRSAPER PSAAEAIKVC GSYLLQRGMD SRKILAHLRA
601SICQVYQEEE TISLQNAFSV VELKPSVAPA PEPSPGFLPV NSDTGLVAVP GPVPGQHPCG
661EEATQLPAAF TSEATHFKPI VLAQNASVAR DQPALAQEES EERGGQREGE GEEKLSLEAH
721AGSPSLKTPD GPVPGPGPQG AAPEPLGASV QRDSAQGRPC PPPQAPANQP EGASSAAPGS
781PVPAPPTKAS ALPVEQGPAE PIPPGVASGG LRPDALGPTT AHHGPRHPPK PPRSKATERP
841GQEPEGPGAT PAGERDDQSP DSVPERPRPA DRRLCLPCVD ASPLPGRTAC PSLQEATRLI
901QEEFAFDGYL DNGLEALIMG EYIFALKDLT FATFCGAISE KFCDLYWDEK LLQNLFKVVN
961GQASPSPSTA EEAGSQLEGS QSPRSPSSKR PSLHRLGKEK PAMARTSSRA PCSPTSVSDV
1021DSDALSRGNF EVGFRPQRSV KAERAQQPEA GEDRRPAGGA SDVEAVTRLA RSKGVGPALS
1081PGPAGFQSCS PGWCSAFYEA DCFGADVHNY VKDLGRQQAD GALPDAQSPE LEQQLMMEKR
1141NYRKTLKFYQ KLLQKEKRNK GSDVKTMLSK LKGQLEEMKS RVQFLSLVKK YLQVMYAERW
1201GLEPCTLPVI VNIAAAPCDT LDFSPLDESS SLIFYNVNKH PGGRQKARIL QAGTPLGLMT
1261YLYSSDAFLE GYVQQFLYTF RYFCTPHDFL HFLLDRINST LTRAHQDPTS TFTKIYRRSL
1321CVLQAWVEDC YAVDFPRNSG LLGKLEDFIS SKILPLDGSA KHLLGLLEVG MDRRAEGNPR
1381GTDLENPREA EEDARPFNAL CKRLSEDGIS RKSFPWRLPR GNGLVLPPHK ERPYTIAAAL
1441PKPCFLEDFY GPCAKTSEKG PYFLTEYSTH QLFSQLTLLQ QELFQKCHPV HFLNSRALGV
1501MDKSTAIPKA SSSESLSAKT CSLFLPNYVQ DKYLLQLLRN ADDVSTWVAA EIVTSHTSKL
1561QVNLLSKFLL IAKSCYEQRN FATAMQILSG LEHLAVRQSP AWRILPAKIA EVMEELKAVE
1621VFLKSDSLCL MEGRRFRAQP TLPSAHLLAM HIQQLETGGF TMTNGAHRWS KLRNIAKVVS
1681QVHAFQENPY TFSPDPKLQS YLKQRIARFS GADISTLAAD SRANFHQVSS EKHSRKIQDK
1741LRRMKATFQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1749 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.