Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KLK8 / Kallikrein 8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127364-100
Catalog Number: LS-G127364
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kallikrein 8(KLK8) (NM_144505), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HK8 | Kallikrein 8 | Kallikrein-related peptidase 8 | KLK8 protein type 1 | KLK8 protein type 2 | Neuropsin | Neuropsin type 1 | HNP | NP | NRPN | Ovasin | Kallikrein-8 | PRSS19 | Serine protease TADG-14 | KLK8 | Neuropsin type 2 | Serine protease 19 | TADG14
- Origin Species
- Human
- NCBI GenBank
- NM_007196
- SwissProt
- O60259
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRPRPRAAK TWMFLLLLGG AWAACGSLDL LTKLYAENLP CVHLNPQWPS QPSHCPRGWR61SNPLPPAAGH SRAQEDKVLG GHECQPHSQP WQAALFQGQQ LLCGGVLVGG NWVLTAAHCK121KPKYTVRLGD HSLQNKDGPE QEIPVVQSIP HPCYNSSDVE DHNHDLMLLQ LRDQASLGSK181VKPISLADHC TQPGQKCTVS GWGTVTSPRE NFPDTLNCAE VKIFPQKKCE DAYPGQITDG241MVCAGSSKGA DTCQGDSGGP LVCDGALQGI TSWGSDPCGR SDKPGVYTNI CRYLDWIKKI301IGSKGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
305 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.