Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KLK4 / Kallikrein 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116318-100
Catalog Number: LS-G116318
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kallikrein 4(KLK4) (NM_004917), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AI2A1 | ARM1 | Androgen-regulated message 1 | EMSP | EMSP1 | Kallikrein-like protein 1 | Kallikrein | Prostase | KLK4 | PRSS17 | PSTS | Kallikrein 4 | Kallikrein-4 | Kallikrein-related peptidase 4 | KLK-L1 | Serine protease 17
- Origin Species
- Human
- NCBI GenBank
- NM_004917
- SwissProt
- Q9Y5K2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 27.03 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATAGNPWGW FLGYLILGVA GSLVSGSCSQ IINGEDCSPH SQPWQAALVM ENELFCSGVL61VHPQWVLSAA HCFQNSYTIG LGLHSLEADQ EPGSQMVEAS LSVRHPEYNR PLLANDLMLI121KLDESVSESD TIRSISIASQ CPTAGNSCLV SGWGLLANGR MPTVLQCVNV SVVSEEVCSK181LYDPLYHPSM FCAGGGHDQK DSCNGDSGGP LICNGYLQGL VSFGKAPCGQ VGVPGVYTNL241CKFTEWIEKT VQASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
254 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.