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KLHL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102212-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL6 (NM_130446), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kelch-like protein KLHL6 | Kelch-like protein 6 | Kelch-like 6 (Drosophila) | KLHL6
Origin Species
Human
NCBI GenBank
NM_130446
SwissProt
Q8WZ60

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDVVEKSLE GPLAPSTDEP SQKTGDLVEI LNGEKVKFDD AGLSLILQNG LETLRMENAL
61TDVILCVDIQ EFSCHRVVLA AASNYFRAMF CNDLKEKYEK RIIIKGVDAE TMHTLLDYTY
121TSKALITKQN VQRVLEAANL FQFLRMVDAC ASFLTEALNP ENCVGILRLA DTHSLDSLKK
181QVQSYIIQNF VQILNSEEFL DLPVDTLHHI LKSDDLYVTE EAQVFETVMS WVRHKPSERL
241CLLPYVLENV RLPLLDPWYF VETVEADPLI RQCPEVFPLL QEARMYHLSG NEIISERTKP
301RMHEFQSEVF MIIGGCTKDE RFVAEVTCLD PLRRSRLEVA KLPLTEHELE SENKKWVEFA
361CVTLKNEVYI SGGKETQHDV WKYNSSINKW IQIEYLNIGR WRHKMVVLGG KVYVIGGFDG
421LQRINNVETY DPFHNCWSEA APLLVHVSSF AATSHKKKLY VIGGGPNGKL ATDKTQCYDP
481STNKWSLKAA MPVEAKCINA VSFRDRIYVV GGAMRALYAY SPLEDSWCLV TQLSHERASC
541GIAPCNNRLY ITGGRDEKNE VIATVLCWDP EAQKLTEECV LPRGVSHHGS VTIRKSYTHI
601RRIVPGAVSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.