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KLHL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103487-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL5 (NM_199039), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Berg36 | Kelch-like 5 (Drosophila) | KLHL5 | Kelch-like protein 5 | Kelch (Drosophila)-like 5
Origin Species
Human
NCBI GenBank
NM_015990
SwissProt
Q96PQ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MNVIYFPLHI FVVYSRAYTS LVLVGCTNLC AVLFARCLDD HLVSLRMSGS RKEFDVKQIL
61KIRWRWFGHQ ASSPNSTVDS QQGEFWNRGQ TGANGGRKFL DPCSLQLPLA SIGYRRSSQL
121DFQNSPSWPM ASTSEVPAFE FTAEDCGGAH WLDRPEVDDG TSEEENESDS SSCRLVLSSV
181SDYFAAMFTN DVREARQEEI KMEGVEPNSL WSLIQYAYTG RLELKEDNIE CLLSTACLLQ
241LSQVVEACCK FLMKQLHPSN CLGIRSFADA QGCTDLHKVA HNYTMEHFME VIRNQEFVLL
301PASEIAKLLA SDDMNIPNEE TILNALLTWV RHDLEQRRKD LSKLLAYIRL PLLAPQFLAD
361MENNVLFRDD IECQKLIMEA MKYHLLPERR PMLQSPRTKP RKSTVGTLFA VGGMDSTKGA
421TSIEKYDLRT NMWTPVANMN GRRLQFGVAV LDDKLYVVGG RDGLKTLNTV ECYNPKTKTW
481SVMPPMSTHR HGLGVAVLEG PMYAVGGHDG WSYLNTVERW DPQARQWNFV ATMSTPRSTV
541GVAVLSGKLY AVGGRDGSSC LKSVECFDPH TNKWTLCAQM SKRRGGVGVT TWNGLLYAIG
601GHDAPASNLT SRLSDCVERY DPKTDMWTAV ASMSISRDAV GVCLLGDKLY AVGGYDGQAY
661LNTVEAYDPQ TNEWTQVAPL CLGRAGACVV TVKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

694 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.