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KLHL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107966-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL4 (NM_057162), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKELCHL | Kelch-like 4 (Drosophila) | Kelch (Drosophila)-like 4 | Kelch-like protein 4 | KHL4 | KIAA1687 | KLHL4
Origin Species
Human
NCBI GenBank
NM_019117
SwissProt
Q9C0H6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVSGKKEFD VKQILRLRWR WFSHPFQGST NTGSCLQQEG YEHRGTPVQG RLKSHSRDRN
61GLKKSNSPVH HNILAPVPGP APAHQRAVQN LQQHNLIVHF QANEDTPKSV PEKNLFKEAC
121EKRAQDLEMM ADDNIEDSTA RLDTQHSEDM NATRSEEQFH VINHAEQTLR KMENYLKEKQ
181LCDVLLIAGH LRIPAHRLVL SAVSDYFAAM FTNDVLEAKQ EEVRMEGVDP NALNSLVQYA
241YTGVLQLKED TIESLLAAAC LLQLTQVIDV CSNFLIKQLH PSNCLGIRSF GDAQGCTELL
301NVAHKYTMEH FIEVIKNQEF LLLPANEISK LLCSDDINVP DEETIFHALM QWVGHDVQNR
361QGELGMLLSY IRLPLLPPQL LADLETSSMF TGDLECQKLL MEAMKYHLLP ERRSMMQSPR
421TKPRKSTVGA LYAVGGMDAM KGTTTIEKYD LRTNSWLHIG TMNGRRLQFG VAVIDNKLYV
481VGGRDGLKTL NTVECFNPVG KIWTVMPPMS THRHGLGVAT LEGPMYAVGG HDGWSYLNTV
541ERWDPEGRQW NYVASMSTPR STVGVVALNN KLYAIGGRDG SSCLKSMEYF DPHTNKWSLC
601APMSKRRGGV GVATYNGFLY VVGGHDAPAS NHCSRLSDCV ERYDPKGDSW STVAPLSVPR
661DAVAVCPLGD KLYVVGGYDG HTYLNTVESY DAQRNEWKES MQELLQNFYT TQKLKETLGH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

720 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.