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KLHL36 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL36 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109818-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C16orf44(KLHL36) (NM_024731), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kelch-like 36 (Drosophila) | C16orf44 | Kelch-like protein 36 | KLHL36
Origin Species
Human
NCBI GenBank
NM_024731
SwissProt
Q8N4N3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMEGSRQTRV SRPYKISESS KVYRWADHSS TVLQRLNEQR LRGLFCDVVL VADEQRVPAH
61RNLLAVCSDY FNSMFTIGMR EAFQKEVELI GASYIGLKAV VDFLYGGELV LDGGNIDYVL
121ETAHLLQIWT VVDFCCEYLE QEVSEDNYLY LQELASIYSL KRLDAFIDGF ILNHFGTLSF
181TPDFLQNVSM QKLCVYLSSS EVQRECEHDL LQAALQWLTQ QPEREAHARQ VLENIHFPLI
241PKNDLLHRVK PAVCSLLPKE ANCEGFIEEA VRYHNNLAAQ PVMQTKRTAL RTNQERLLFV
301GGEVSERCLE LSDDTCYLDA KSEQWVKETP LPARRSHHCV AVLGGFIFIA GGSFSRDNGG
361DAASNLLYRY DPRCKQWIKV ASMNQRRVDF YLASIEDMLV AIGGRNENGA LSSVETYSPK
421TDSWSYVAGL PRFTYGHAGT IYKDFVYISG GHDYQIGPYR KNLLCYDHRT DVWEERRPMT
481TARGWHSMCS LGDSIYSIGG SDDNIESMER FDVLGVEAYS PQCNQWTRVA PLLHANSESG
541VAVWEGRIYI LGGYSWENTA FSKTVQVYDR EADKWSRGVD LPKAIAGGSA CVCALEPRPE
601DKKKKGKGKR HQDRGQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

616 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.