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KLHL35 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL35 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129050-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL35 (NM_001039548), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
26597 | Kelch-like 35 (Drosophila) | Kelch-like protein 35 | KLHL35
Origin Species
Human
SwissProt
Q6PF15

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRQGHAPEES EPGCEAPCAG PCHAQRVLQA LNAYRRSGTL TDVVLRAGGR DFPCHRAALS
61AGSAYFRSLF AAGRPERGPA VVPVVPVAPE APGTSPAGAA AALAVVLDYV YGAGVRLRAE
121DEAAAVLALA ERLGVAGLRE ACVRFLEGRL RAANSLALRR VAAAFSLAPL AERCGRVLRQ
181AFAEVARHAD FLELAPDEVV ALLADPALGV AREEAVFEAA MRWVRHDAPA RRGQLRRLLE
241HVRLPLLAPA YFLEKVEADE LLQACGECRP LLLEARACFI LGREAGALRT RPRRFMDLAE
301VIVVIGGCDR KGLLKLPFAD AYHPESQRWT PLPSLPGYTR SEFAACALRN DVYVSGGHIN
361SHDVWMFSSH LHTWIKVASL HKGRWRHKMA VVQGQLFAVG GFDGLRRLHS VERYDPFSNT
421WAAAAPLPEA VSSAAVASCA GKLFVIGGAR QGGVNTDKVQ CFDPKEDRWS LRSPAPFSQR
481CLEAVSLEDT IYVMGGLMSK IFTYDPGTDV WGEAAVLPSP VESCGVTVCD GKVHILGGRD
541DRGESTDKVF TFDPSSGQVE VQPSLQRCTS SHGCVTIIQS LGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

583 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.