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KLHL33 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL33 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124188-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL33 (NM_001109997), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLSGMRESQ GTEVSLRTIS TQDLRLLVSF AYSGVVRARW PGLLRAAQAA LQYQSSSCLD
61LCQKGLARGL SPARCLALFP MAEAPGLERL WSKARHYLLT HLPAVALCPA FPSLPAACLA
121ELLDSDELHV QEEFEAFVAA RCWLAANPET QESEAKALLR CVRFGRMSTR ELRRVRAAGL
181LPPLTPDLLH QLMVEADVPG QERRREPDRA LVVIGGDGLR PDMALRQPSR AVWWARAFRC
241GVGLVRTVEW GQLPALPAPG RFRHGAASLA GSELYVCGGQ DFYSHSNTLA STLRWEPSQE
301DWEEMAPLSQ ARSLFSLVAL DGKLYALGGR HNDVALDSVE TYNPELNVWR PAPALPAPCF
361AHAAAILEGQ LYVSGGCGGT GQYLASLMHY DPKLEKPGTF LSPMGVPRAG HVMAALGGRL
421YVAGGLGETE DLLSFEAYEL RTDSWTHLAP LPSPHVGAAS AVLQGELLVL GGYSHRTYAL
481SHLIHAYCPG LGRWLCLGTL PRPRAEMPAC ILTLPAVQHI ALVPTPHQTK PAG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

533 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.