LSBio
KLHL32 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL32 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KLHL32 antibodies →
$725.00each
Catalog Number: LS-G108103-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL32 (NM_052904), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BKLHD5 | DJ21F7.1 | KLHL32 | Kelch-like 32 (Drosophila) | Kelch-like protein 32 | KIAA1900 | RP1-39B17.1
Origin Species
Human
SwissProt
Q96NJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSERCLSIQ EMLTGQRLCH SESHNDSVLA ALNQQRSDGI LCDITLIAEE QKFHAHKAVL
61AACSDYFRAM FSLCMVESGA DEVNLHGVTS LGLKQALEFA YTGQILLEPG VIQDVLAAGS
121HLQLLELLNL CSHYLIQELN SFNYLDLYRL ADLFNLTLLE KAVIDFLVKH LSELLKSRPE
181EVLTLPYCLL QEVLKSDRLT SLSEEQIWQL AVRWLEHNCH YQYMDELLQY IRFGLMDVDT
241LHTVALSHPL VQASETATAL VNEALEYHQS IYAQPVWQTR RTKPRFQSDT LYIIGGKKRE
301VCKVKELRYF NPVDQENALI AAIANWSELA PMPVGRSHHC VAVMGDFLFV AGGEVEHASG
361RTCAVRTACR YDPRSNSWAE IAPMKNCREH FVLGAMEEYL YAVGGRNELR QVLPTVERYC
421PKKNKWTFVQ SFDRSLSCHA GYVADGLLWI SGGVTNTAQY QNRLMVYEPN QNKWISRSPM
481LQRRVYHSMA AVQRKLYVLG GNDLDYNNDR ILVRHIDSYN IDTDQWTRCN FNLLTGQNES
541GVAVHNGRIY LVGGYSIWTN EPLACIQVLD VSREGKEEVF YGPTLPFASN GIAACFLPAP
601YFTCPNLQTL QVPHHRIGTI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.